Theerlenmeyer shake flaskke setshelo se khethehileng sa setso bakeng sa setso sa sele se emisitsoeng, hape se ka sebelisoa ho lokisa, ho kopanya le ho boloka mecha ea litaba e fapaneng.Tloaelo ena e sebelisoa ha ho lengoa lisele tsa Jurkat.

Jurkat cell line e tsoa maling a peripheral a moshanyana ea lilemo li 14 mme ke sele e emisitsoeng.Lisele tsa lisele tse tsoang ho Jurkat tse haelloang ke liphatsa tsa lefutso tse itseng li se li fumaneha libankeng tsa cell culture.Immortalized human T lymphocyte lines haholo-holo e sebelisetsoang ho ithuta acute T cell leukemia, T cell signaling, le li-receptor tse fapa-fapaneng tsa chemokine tse kotsing ea ho kena ka kokoana-hloko, haholo-holo polelo ea HIV.E na le mefuta e mengata haholo ea likopo tsa lipatlisiso tsa likokoana-hloko, tse kang tšebeliso ea lisele tsa Jurkat ho ithuta M1-RNA ea ribonuclease P, le thuto ea M1-RNA ea anti-MHC class II transcriptional activator (CIITA) ho thibela. polelo ea limolek'hule tsa sehlopha sa MHC sa II holim'a sele.

Ha u hlaolela lisele tsa Jurkat ka erlenmeyer shake flasks, RPMI1640 medium, 10% FBS ea hlokahala;mocheso o laoloa ho 37 ° C, 5% carbon dioxide, PH bohlokoa 7.2-7.4, aseptic kamehla mocheso setso.Hlakola 'me u bolaee likokoana-hloko ka 75% ea joala pele u fetisetsa bencheng e hloekileng ka ho fetisisa, u ntše seleng ea cryovial ho tloha tanka ea metsi ea naetrojene, hang-hang u e behe ka bateng ea metsi ea 37 ° C ebe u sisinya seleng ea cryotube ka potlako ho e qhibiliha ka potlako.Joale, ka mor'a centrifugation, pipetting le ho kopanya, joalo-joalo, e ne e kenngoa ka seqhoqhoa sa sele bakeng sa ho lema.

Lisele li ameha haholo tikolohong.Ha u hlaolela lisele tsa Jurkat ka li-flask tsa erlenmeyer cell shake, bohloeki ba motho bo lokela ho etsoa hantle, ho lokela ho sebelisoa li-reagents tse nyopa, 'me melao-motheo ea ts'ebetso ea aseptic e lokela ho lateloa ho qoba ho hlahisa libaktheria le ho ama kholo ea lisele.