Hang ha lisele tse re setso ho tsona

feme ea liseleli silafetse, boholo ba tsona ho thata ho li tšoara.Haeba lisele tse silafetseng li le bohlokoa ’me ho le thata ho li fumana hape, ho ka sebelisoa mekhoa e latelang ho li ntša.

1. Sebelisa lithibela-mafu

Lithibela-mafu li sebetsa ho feta ho bolaea libaktherialifeme tsa lisele.Motsoako oa meriana o sebetsa ho feta meriana feela.Meriana e thibelang e sebetsa ho feta meriana ka mor'a ho silafala.Meriana e thibelang hangata e sebelisa lithibela-mafu tse peli (penicillin 100u/mL hammoho le streptomycin 100μg/mL).Kamora ho silafatsoa, ​​mokhoa oa ho hloekisa o hloka ho ba makhetlo a 5 ho isa ho a 10 ho feta tekanyo e tloaelehileng.Setlhare se lokela ho sebelisoa lihora tse 24 ho isa ho tse 48 kamora ho eketsoa, ​​ebe se nkeloa sebaka ke mokhoa o tloaelehileng.Mokelikeli oa setso.Mokhoa ona o ka sebetsa maemong a pele a tšoaetso.Ntle le penicillin le streptomycin, lithibela-mafu tse sebelisoang li ka boela tsa kenyelletsa gentamicin, kanamycin, polymyxin, tetracycline, nystatin, joalo-joalo. Hangata ho sebelisoa ke 400 ho 800 μg / mL kanamycin kapa 200 μg / mL tetracycline.Mokhoa ona o fetoloa matsatsing a mang le a mang a 2 ho isa ho a 3 mme o fetisetsoa melokong e 1 ho isa ho e 2 bakeng sa kalafo.Lilemong tsa morao tjena, ho tlalehiloe hore 4-fluoro, 2-hydroxyquinoline (Ciprofloxacin, Cip), Pleu-romutilin derivative (Pleu-romutilin derivative, BM-Cyclin2: BM-1 le tetracycline derivative (BM-2)) Lithibela-mafu li teng. e atlehang ho bolaea mycoplasma ha e sebelisoa e le mong kapa e kopane.Lithibela-mafu tsena tse tharo kaofela li lokiselitsoe ho ba litharollo tse tsepamisitsoeng tsa 250X ho PBS 'me li bolokoa ho -20°C hore li sebelisoe hamorao.Tšebeliso ea mahloriso Cip ke 10 μg/mL, BM-1 ke 10 μg/mL, le BM-2 ke 5μg/mL.Ha u sebelisa, qala ka ho lakatsa mokhoa oa setso o silafetseng, eketsa RPMI1640 setso se nang le BM-1, ebe u lakatsa setso sa setso ka mor'a matsatsi a 3, eketsa RPMI1640 setso se nang le BM-2, le setso bakeng sa matsatsi a 4, joalo-joalo ka matsatsi a 3 a latellanang. .pota-pota, ho fihlela ho pakoa ke 33258 fluorescent staining microscopy hore mycoplasma e felisitsoe, joale mokhoa o tloaelehileng oa setso o eketsoa bakeng sa setso le ho feta makhetlo a 3-4.

2. Kalafo ea ho futhumatsa

Ho kenya setso sa linama tse silafetseng ka 41°C ka lihora tse 18 ho ka bolaea mycoplasma, empa ho na le litlamorao tse mpe liseleng.Ka hona, tlhahlobo ea pele e lokela ho etsoa pele ho phekolo ho hlahloba nako ea ho futhumatsa e ka bolaeang mycoplasma ho isa tekanyong e kholo le ho ba le tšusumetso e fokolang liseleng.Mokhoa ona ka linako tse ling ha o tšepahale.Haeba e phekoloa ka lithethefatsi pele ebe e futhumatsoa ho 41 ° C, phello e tla ba molemo.

3. Sebelisa serum e khethehileng ea mycoplasma

Tšilafalo ea Mycoplasma e ka tlosoa ka 5% ea mmutla mycoplasma immune serum (hemagglutination titer 1:320 kapa ka holimo).Hobane lesole la 'mele le ikhethileng le ka thibela kholo ea mycoplasma, e fetoha e mpe matsatsi a 11 kamora kalafo ea antiserum mme e lula e le mpe likhoeli tse 5 hamorao.e mpe.Leha ho le joalo, mokhoa ona o thata haholo ebile ha o bonolo ebile ha o na chelete e ngata joaloka ho sebelisa lithibela-mafu.

4. Mekhoa e meng

Ntle le mekhoa e boletsoeng ka holimo ea ho tlosa tšilafalo, ho boetse ho na le mekhoa ea inoculation le sterilization ho liphoofolo, mekhoa ea macrophage phagocytosis, mekhoa ea ho eketsa bromouracil ho silafalitsoeng.libotlolo tsa setsoebe o li khantša ka leseli, le mekhoa ea ho sefa, joalo-joalo, empa kaofela li thata ebile ha li na thuso.Ka hona, hang ha tšilafalo ea mycoplasma e etsahala, ntle le haeba e le ea bohlokoa ka ho khetheha, hangata e lahloa le ho tsosolosoa hape.

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