Ho na le likaroloana tse ngata tsa lisele ka mor'a lysis ea mechine ea ho emisoa ha sele.Joang ho tlosa likhechana tsee?Ha re shebeng mekhoa e fapaneng:

1. Sebelisa mokhoa oa ho hlapolla.Ha lisele li hlapolloa, le tsona li ka ata, li tla ata le ho feta, 'me lisele tsa lisele li tla fokotseha ka ho tšoanang.
2. Ho boetse ho na le bolulo ba tlhaho.Lisele li lula kapele ho feta likhechana tse ngata: : Tsamaisa ho fanyeha ha sele hotube ea centrifuge, 'me ha boholo ba lisele li teba, tharollo e ka holimo e ka monngoa, ebe e kenngoa ho tharollo ea setso ho emisa lisele.Mokhoa ona o ka sebelisoa khafetsa, ha nako le nako u ka bona hore na o fihlela litlhoko tsa hau tlas'a microscope.
3. centrifuge e lebelo le tlasee ka tlosa maloanlahla, ka kakaretso 700g,5mins
4. Ha o etsa centrifuging, tlas'a boemo ba hore palo ea lisele e lekane, fokotsa nako ea centrifugation, e kang 5min, 1000rpm, ho e-na le 3min, 1000rpm, 'me u tlose matla a maholo, hobane necrosis le litšila li atisa ho ba holimo!Opereishene ena e hloka ho etsoa hanngoe feela nakong ea ts'ebetso ea ho hlatsoa pele e kenngoa!

Le hoja ho na le mekhoa e mengata ea ho tlosa litšila tsa lisele, u tla fumana hore centrifugation ke e 'ngoe ea mekhoa e sebetsang ka ho fetisisa!

'Me kaha lisele tsa semela le lisele li na le mabota a lisele tse entsoeng ka selulose, hemicellulose le pectin, hangata hoa hlokahala ho sila lehlabathe la quartz kapa phofo ea khalase ka tharollo e loketseng ea ho ntša kapa ho tšoara cellulase ho finyella morero.Karohano ea lisele tsa baktheria e thata le ho feta, hobane skeleton eohle ea baktheria cell lerako ha e le hantle ke tlamo e kopanetsoeng ea peptidoglycan cystic macromolecules, e thata haholo.Mekhoa e tloaelehileng ea ho pshatla marako a lisele tsa baktheria e kenyelletsa ho pshatla ha ultrasonic, ho sila lehlabathe, extrusion e phahameng ea khatello kapa phekolo ea lysozyme.Kamora hore lisele le lisele li robehe, ho khethoa buffer e loketseng ho ntša protheine e batloang.Lisebelisoa tse sa tsitsang joalo ka likhechana tsa lisele li tlosoa kecentrifugekapa tlhoekiso.

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